In the present study a total of five bacterial strains were used to
control isolated pathogens of which two were plant rhizospheric
bacteria viz. Burkholderia cenocepacia VBC7 (NCBI GenBank Accession
No: HM042678) and Pseudomonas poae VBK1 (NCBI GenBank
Accession No: GU384238) (Mukhopadhyay, 2010) and another
three different strains (LABW1, LABW3 and LABW4) of Lactococcus
lactis subsp. lactis isolated in our laboratory and identified following
16S rDNA sequence homology. The two rhizobacterial strains B.
cenocepacia VBC7 and P. poae VBK1 were isolated from the rhizospheric
soil of Chick pea (Cicer arietinum L.) and mung bean (Vigna
radiata (L.) R. Wilczek) respectively on nitrogen free Burk’s medium
and were selected on the basis of their plant growth promoting
properties. On the other hand all of the lactic acid bacterial
strains were isolated from good quality fermented milk products
(whey) on de Man Rogosa and Sharpe (MRS) medium and were
selected for their very good antimicrobial activities. Antifungal
activities of these five bacterial strains were checked against isolated
pathogens by dual culture overlay assay (Magnusson and
Schnürer, 2001). The antifungal activities of the cell free supernatants
of the bacterial strains were also checked against isolated
pathogens by agar well diffusion method (Fernández-Garayzábal
et al., 1992). Here both boiled and unboiled cultures were tested.
Inhibition of percent spore germination of VBAM1 by different