Then,
salt was added to the withered vegetables (2.5 %, w/w) and
rubbed gently by hand, similar to the traditional process.
Subsequently, the leaves were immersed in coconut water
containing 1 % (v/v) of the standard inoculum, at a ratio
of 1:5 (w/v). Each product was packed in 360-mL glass jar
and incubated in the dark at a room temperature of 22 °C
for 7 days. Each treatment was carried out in triplicate.