RBCs and PLTs are counted in a dedicated channel using the direct current detection method with hydrodynamic focusing technology to minimize coincidence or recirculation. Automatic discriminators separate the two cell populations based on complex algorithms. The intensity of the electronic pulse from each analyzed RBC is proportional to the cell volume. The hematocrit (HCT) is directly determined based on the count and volume detection of each individual RBC. Even with samples at extremely low or unusually high concentrations, the Sysmex cell counters analyze RBCs and platelets with uncompromised precision and accuracy