The indigenous Indonesian bacterial isolates
viz. B. firmus (E 65), P. aeruginosa (C 32b), S.
marcescens (E 31), and B. cereus (II.14) were
obtained from the microbial gene banks culture
collection of Microbiology Laboratory (BiogenCC),
and Department of Biology, Faculty of
Mathematics and Natural Sciences (IPB). Stock
cultures of bacteria were prepared using Nutrient
Broth (NB) medium. Colonies were selected,
purified and used for further studies.