2.1. Plant material
All types of embryos used in the study were of
the same origin, i.e. a highly inbred line of cv.
Borszczagowski. Zygotic embryos were collected
from ethanol sterilised fruits from greenhouse
grown, hand pollinated plants. ZE were collected
on the following days after pollination (DAP): 21
(Fig. 1A), 24, 28, 35, and 42. Samples of ZE
dissected at 42 DAP and stored for further 14 days
(for simplicity designated as a 56 DAP) were also
included in the study. Due to the delay and abnormalities
in the development the ZE of 3n line were
collected only at 35 DAP (Fig. 1B) and 56 DAP.
Whole ovules, testa and ovary tissues surrounding
the seeds were also collected at certain time points.
Somatic embryos of the same diploid inbred line
were collected from established, embryogenic cell
suspension cultures.