The test was done according to the methods
followed by Thenmozhi et al., method [13].
Four drops of concentrated sample were put near
the adage of aluminum oxide plate of thin layer
chromatography(TLC)plate , then put the TLC
plate in the jar with three different solvents
system Toluene :Acetone :Formic acid
(11:6:1),which act as mobile phase, then the
transferred spot was tested under UV. Light, the
appearance of purple to violet color was
indicated the coumarines were presence in the
plant.