Khao Sok National Park is a virgin forest where various types of plant life can be found. This includes a number of very rare and sacred tree species such as the Neobalanocarpus heimil. A few other plants that can be seen in this park include the following: Genus Hoper, Chisocheton and Anisoptera etc., as well as small bushes such as the Rafflesia kerrii, palm, rattan, betel palm, and various types of bamboo.
The park serves as a home for a wide variety of wildlife such as the gaur, banteng, sambar deer, bear, Malayan tapir, macaque, gibbon, serow, mouse deer, porcupine, marbled cat, wild boar, and Asian wild dog. It also acts as the natural habitat for several for several bird species.