Laccases from Trametes hirsuta (100 U/mL, 693 mg/mL) were used at pH 5
(0.1MNa–acetate buffer) to perform the enzymatic experiments. The flavonoids
(Fig. 1) were purchased with high purity from Aldrich and used as supplied.
The bio desized woven cotton fabric having 80/65warp/weft cm−1 was
supplied from project ‘ViaBio’. The enzymatically desized 100% cotton was
scoured with 1% NaOH and 0.2% Lutensol AT 25 solution for 1 h at boiling
temperature. [18]. Then it was bleached with 5% hydrogen peroxide, 1% commercial
sodium hydroxide,3%sodium silicate (w/w of fabric) and 0.1% Lutensol
AT 25 solution for 100 min at boiling temperature. The other chemicals were
purchased from Aldrich at degree of purity higher than 95%.