tap water, (1000 ml)] containing 50 μg chloramphenicol/ml. The
isolated strain of yeast was maintained on YPD slopes.
A taxonomical study of the yeast was carried out by NCIMB
Japan, Co., Ltd. (Shimizu, Japan). For a comparative study, the
industrial wine yeast S. cerevisiae W-4 was purchased from the
Brewing Society of Japan (Tokyo, Japan) was used.