Duration of Tourniquet Application
The application of the tourniquet, by reducing the pres- sure below the systolic pressure, maintains the effective filtration pressure within the capillaries.2 As a result, small molecules and fluid are transferred from the intravasal space to the interstitium. Application of the tourniquet for longer than 1 minute and up to 3 minutes can result in hemoconcen- tration, causing an increase in the concentration of large molecules that are unable to penetrate the capillary wall (Table 5).2,8,24,41
The venous stasis that results from prolonged tourniquet application promotes anaerobic glycolysis with the accumu- lation of plasma lactate and a reduction in blood pH.8 The hypoxic effect drives potassium out of the cell causing a spurious increase in the serum potassium level. This effect can be accentuated if the phlebotomist requests the subject to
Duration of Tourniquet Application
The application of the tourniquet, by reducing the pres- sure below the systolic pressure, maintains the effective filtration pressure within the capillaries.2 As a result, small molecules and fluid are transferred from the intravasal space to the interstitium. Application of the tourniquet for longer than 1 minute and up to 3 minutes can result in hemoconcen- tration, causing an increase in the concentration of large molecules that are unable to penetrate the capillary wall (Table 5).2,8,24,41
The venous stasis that results from prolonged tourniquet application promotes anaerobic glycolysis with the accumu- lation of plasma lactate and a reduction in blood pH.8 The hypoxic effect drives potassium out of the cell causing a spurious increase in the serum potassium level. This effect can be accentuated if the phlebotomist requests the subject to
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