The resultswere presented asmean±SE of three replicates. All data
were subjected to one-way analysis of variance (ANOVA).When overall
differenceswere significant (P b 0.05), Tukey's testwas used to compare
the mean values among the treatments. Statistical analysis was
performed using the SPSS 18.0 for Windows (SPSS, Chicago, IL, USA).
Dietary Zn requirement of tilapia was estimated by broken-line regression
analysis (Robbins et al., 2006) using both weight gain and bone Zn
concentration.