Among all organic acids, levulinic acid was found not to be as effective as other organic acids when applied with 5 g/L EG against APC on inoculated beef trimming. When treated with 30 g/L levulinic acid, APC population of the inoculated beef trimming reduced (P < 0.05) by 0.96 log10 CFU/g. A combination of 30 g/L levulinic acid with 5 g/L EG, reduced (P < 0.05) 0.5 log10 CFU/g of APC on inoculated beef trimmings. Among all antimicrobials used in this study, treatment of inoculated beef trimmings with 0.2 g/L peroxyacetic acid exhibited reduction of APC population by 0.64 log10 CFU/g, while treatment of inoculated beef trimming with 0.2 g/L peroxyacetic acid with 5 g/L EG reduced (P < 0.05) 0.41 log10 CFU/g as compared with control (Fig. 6).