Accumulated evidence suggests that the growth hormone ( GH ) gene plays a physiological role in the control of reproductive
function. Here, we examined the correlation between egg-laying traits and GH gene polymorphisms and expression patterns in the
muscovy duck ( Cairina moschata ). PCR single-strand conformation polymorphism was used to identify polymorphisms in intron
3 of GH . One single nucleotide polymorphism (g.3270 A G) was detected by sequencing, and the frequencies of the A and G
alleles in the population were 0.65 and 0.35, respectively. A comparison test showed that the AA genotype group had more
consecutive laying days and more eggs at 300 days than the GG genotype group (P 0.05); however, there was no signifi cant
difference for the age at fi rst laying (P 0.05). Such a signifi cant correlation between GH polymorphisms and egg-laying
performance suggested that GH could be a candidate locus affecting the laying trait in muscovy duck. Furthermore, real-time
fl uorescent quantitative PCR demonstrated that GH is expressed in all selected tissues, but is highly expressed in the hypothalamic –
pituitary – gonadal axis and heart. This unique expression pattern suggested that GH may exert its local physiological function
through the autocrine or paracrine pathway during gonad development and growth in the muscovy duck. The data presented in this
paper revealed GH polymorphisms and expression patterns in the muscovy duck and indicated a potential regulatory effect of GH
on reproduction.
Accumulated evidence suggests that the growth hormone ( GH ) gene plays a physiological role in the control of reproductivefunction. Here, we examined the correlation between egg-laying traits and GH gene polymorphisms and expression patterns in themuscovy duck ( Cairina moschata ). PCR single-strand conformation polymorphism was used to identify polymorphisms in intron3 of GH . One single nucleotide polymorphism (g.3270 A G) was detected by sequencing, and the frequencies of the A and Galleles in the population were 0.65 and 0.35, respectively. A comparison test showed that the AA genotype group had moreconsecutive laying days and more eggs at 300 days than the GG genotype group (P 0.05); however, there was no signifi cantdifference for the age at fi rst laying (P 0.05). Such a signifi cant correlation between GH polymorphisms and egg-layingperformance suggested that GH could be a candidate locus affecting the laying trait in muscovy duck. Furthermore, real-timefl uorescent quantitative PCR demonstrated that GH is expressed in all selected tissues, but is highly expressed in the hypothalamic –pituitary – gonadal axis and heart. This unique expression pattern suggested that GH may exert its local physiological functionthrough the autocrine or paracrine pathway during gonad development and growth in the muscovy duck. The data presented in thispaper revealed GH polymorphisms and expression patterns in the muscovy duck and indicated a potential regulatory effect of GHon reproduction.
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