Callus induction:
Nucellus tissues (Fig. 1a) were cultured on
four culture media, basically all contained MS
salts and vitamins (Murashige and Skoog,
1962). The first medium, called M1 consisted
of MS medium supplemented with 500 mg l-1
malt extract. At the same time, nucellus tissues
were cultured on MS + 5.0 mg l-1 NAA + 1.0
mg l-1 BA (M2), MS + 5.0 mg l-1 2,4-D + 1.0
mg l-1 BA (M3), MS + 1.0 mg l-1 2,4-D + 5.0
mg l-1 BA (M4). The nucellus halves were
incubated in conical flasks containing five
samples, and five replicates for each medium.
The flasks were incubated in darkness, in a
growth chamber (25±2 ºC) for four weeks.