Thereafter, four replicates of 10μl from each of the selected dilutions were delicately plated onto five different media that were plate count agar (LUQIAO, Beijing, China) for total viable counts; Violet Red Bile Glucose agar (VRBG, Oxoid, CM0485) for Enterobacteriaceae; CFC (Pseudomonads agar base type CM 0559 with selective supplement SR0103; Oxoid) for Pseudomonas spp.