The absorbance at 415nm was measured using a mini 1240 UV–Vis spectrophotometer (Shimadzu) to determine the amount of pNA liberated. The enzyme activity unit (U) was defined as the amount of enzyme that produced 1mol of pNA per min.
The absorbance at 415nmwas measured using a mini 1240 UV–Vis spectrophotometer(Shimadzu) to determine the amount of pNA liberated. Theenzyme activity unit (U) was defined as the amount of enzymethat produced 1mol of pNA per min.