On 18 doi,
all eggs that were designated for injection received their
prespecified treatments using an Intelliject multi-egg
injector (Avitech LLC, Salisbury, MD). Details of the
automated in ovo injection procedures, needle dimensions
and injection depth, egg handling, and verification
of the amnion as the site of injection were as described
by Zhai et al. (2011c). All eggs including those that
belonged to the noninjected control group remained
outside the setter for a maximum of 2 min during the
injection procedure.