One gram of each sample was soaked in distilled water in 250 ml
conical flask for 72 h at 28 ± 2°C. Serial dilution of the steep of each
was carried out up to 10-5
. An aliquot of 0.1 ml of each dilution was
plated on Malt Extract Agar (MEA) plates (5 g/100 ml) using spread
plate technique. The inoculated plates were incubated for 48 h at
28 ± 2°C. Chloramphenicol at the rate of 30 µg/ml was added as an
antimicrobial agent to inhibit all bacteria growth.