Chromatogram of TLC under UV with four major bands and their
respective Rf values are depicted in Fig. 1. Among the major bands,
fraction-2 (F2) appeared as single compound and showed larvicidal
activity. Fraction-4 (F4)wasalso bioactive but appeared as mixtures
of several compounds. Therefore, it was again subjected to repetitive
flash column through which one antilarval compound was
separated.