4. Conclusions
A drop a little more accentuated could be observed in the final pH of the hydrolysates of not toasted
than in the toasted soybean meal. In the three analyzed cases, control, incubation with Alcalase and with
Raquel Strˆher et al. / Procedia Food Science 1 (2011) 463 – 469 469
Flavourzyme, the pH of the suspension of not toasted stayed between 7.0 and 6.5 for up to six hours of
hydrolysis, falling for 6.0 in seven hours and, for larger times for values around 4.5. To the toasted
soybean meal, also in the same three analyzed cases, the pH of the suspension stayed around 6.6 up to
seven hours of hydrolysis, decreasing slightly in eight hours and, for longer times, it was recorded values
around 5.0.
In relation to the soluble proteins, it was observed that the enzymes didn't increase the amount
extracted for the water in the not toasted soybean meal. The highest amount of protein solubilized in the
hydrolysis period was of 2 g/100 mL of suspension, for the control, and in two hours of hydrolysis, what
means that the total amount of protein solubilized was of approximately 3 g/100 mL (60 to 75% of the
present initial protein).
In the experiments using toasted soybean meal, the enzymes increased the amount of soluble protein.
Considering that three hours is the optimum reaction time, the largest amount of protein solubilized in the
three samples was of approximately 2 g/100 mL of suspension, for the samples incubated with the
enzymes, and 1,5 g/100 mL, for the control. That is to say, the total amount of protein solubilized was of
2.15, 2.67 and 2.60 g/100 mL, for the control, incubation with Alcalase and incubation with Flavourzyme,
respectively, meaning that between 40 to 50% of the present initial protein in the toasted soybean meal it
was solubilized in the control, and in the incubations with enzymes those values were superior, between
50 and 65%