AR1-6 liposomes with LEC or PC90G as structural lipid could be prepared by CF, REV, PD, and MFD methods. The obtained liposomes possessed the size range of 0.26–13.83 μm and slightly negative charge of −1.5 to −39.3 mV, whereas the entrapment efficiency and in vitro tyrosinase inhibitory activity were 42.19%–69.08% and 12.06%–25.00%, respectively. Types of lipid and preparation methods significantly influenced the physicochemical properties of liposomes such as vesicle type, size, surface charge, drug entrapment, and biological activity.