A salicylic acid-independent induction of the
antifungal protein PR1 was observed after treatment with
B. subtilis IAB/BS03, with the strongest induction due to
root treatment compared with foliar application. A metabolic
analysis of B. subtilis IAB/BS03 culture broth using
Ultra Performance Liquid Chromatography coupled with
ultraviolet and mass spectrometric detection determined
surfactin and iturin A isomers. These compounds have
been described as antifungal and antibiotic lipopeptides.
The results indicated that B. subtilis IAB/BS03 could be
effectively used as a biocontrol agent.