The MCE by P. oxalicum displayed a dramatic loss in activity after 20 min of incubation at 55 ◦C [20], and that by M. pusillus [53] and Mucor J20 [43] was completely inactivated after heating at 60 ◦C for 20 min and 10 min, respectively. In addition, the crude MCE by Nocardiopsis sp.