Only the analyte of interest is needed for the quantification
using the modified standard addition, therefore, the quantitative
results do not depend on a model-matrix. Recoveries in the recommended
range of 70–120% were observed during the validation of
the method. The addition of the analyte to individual aliquots of
the sample material requires, however, at least four PCR analyses
per sample. Since only a DNA sequence specific for the analyte
needs to be detected, existing real-time PCR methods can be used
for the quantification of the allergen content without
modifications.