Cultivation, harvest and determination of cellulose production
The SH liquid medium was used for cultivating the strains. A
250-mL flask containing 100 mL of SH medium was inoculated with
2%, v/v of starter. A bacterial isolate was inoculated into the SH
medium under static conditions. After finishing the cultivation, the
cellulose was picked from the surface and boiled in 0.1 N NaOH
for 10 min. It was immersed in distilled water for at least 2 h or
overnight, and then rinsed extensively with distilled water before
drying at 60 ◦C for 48 h or until no change of weight. The dried
weight of cellulose was determined.