The analysis of glucose, alcohol and glycerol was made by ELITE LaChrom HPLC system comprised of a VWR HITATCH L-2130 pump, a VWR HITATCH L-2200 autosampler fitted with a 20 μL sample loop, and a VWR HITATCH L-2455 Diode Array detector. Peak data were collected with Agilent EZChrom Elite Client/Server Enterprise Data System Aminex HPX-87X.