Both width and length of guard cells of the
young leaf were significantly affected by colchicine
concentrations (Table 1). The width of guard cell
was the highest at 0 mM colchicine (0.410 mm)
and decreased when higher concentrations of
colchicine were applied. The lowest width at 0.030
mm was obtained from treating with colchicines at
concentration of 7.5 mM.
For the length of guard cell, the longest
of them was found in seedling from treating
inflorescence with 5 mM colchicine at 0.040 mm
while the lowest length was detected at 2.5 mM
(0.034 mm) and 7.5 mM (0.033 mm) colchicine.
The result of ploidy level from leaf of physic
nut detected by flow cytometry was shown in Table