The yeast Saccharomyces cerevisiae was inoculated on Sabouraud
agar consisting of 5.0 g/L peptone, 15.0 g/L glucose and 15.0 g/L
agar and incubated at 30 C for 48 h. Then, three colonies were
transferred from the stock culture to a 500 mL Erlenmeyer flask
containing 200 mL of inoculum medium.