was
extracted using a 5:1 mixture of hexane and ethanol containing 0.5% butylated
hydroxytoluene. The lycopene levels were measured by HPLC on a 4.6 250 mm
COSMOSIL 5 C18-AR II column eluted with a 35:65 mixture of ethanol and
methanol at a flow rate of 0.7 mL/min, with monitoring at 474 nm using an SPDM20
A detector [22]. The lycopene peak was identified by comparison of the
retention time with that of an authentic standard and quantified by comparison
of the peak area to the peak areas on a standard curve.