Organic phases obtained from the two extraction procedures were pooled and cold-evaporated (Evaporator centrifuge model RC1010, Jouan). Then, the organic dry residue was diluted with 75 mL of chloroform for FTIR measurements (Figure 1). Finally, for each sample, we were able to analyze separately the spectral characteristics of the whole bile, the bile aqueous phase, and the bile organic phase.
Organic phases obtained from the two extraction procedures were pooled and cold-evaporated (Evaporator centrifuge model RC1010, Jouan). Then, the organic dry residue was diluted with 75 mL of chloroform for FTIR measurements (Figure 1). Finally, for each sample, we were able to analyze separately the spectral characteristics of the whole bile, the bile aqueous phase, and the bile organic phase.
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