A panel of more than 100 strains of Listeria spp. and non-Listeria was used in order to verify the specificity of the primers and Taqman probe and amplification signals were obtained only when L. monocytogenes DNA and RNAwere loaded in the qPCR mix
A panel of more than 100 strains of Listeria spp. and non-Listeria was used in order to verify the specificity of the primers and Taqman probe andamplification signals were obtained only when L. monocytogenes DNA and RNAwere loaded in the qPCR mix