outhern blot analysis was performed with HindIII-digested DNA
from transgenic and nontransgenic control plants in order to
confirm the integration and to reveal the number of inserted
copies. This enzyme has a single restriction site in the pBI-HRAP
construct used in plant transformation (Fig. 4A). Positive bands
were observed from all eight transgenic lines tested, whereas no
band was observed from the nontransgenic controla.