The protein extract was transferred to a centrifuge bottle (200 ml) and methanol (15 ml) containing 1,6-di-t-buthyl-p-cresol (BHT, 500 mg l1) as an antioxidant was added.
The protein extract was transferred to a centrifugebottle (200 ml) and methanol (15 ml) containing1,6-di-t-buthyl-p-cresol (BHT, 500 mg l1) as an antioxidantwas added.