The supernatant was discarded, and the steeped rice grains were ground with a blender and then passed through a 63 lm screen.
The slurry was allowed to stand at 4 C for 48 h.
The supernatant was removed, and the starch cake was re-suspended in 0.4% sodium hydroxide solution and kept at 4 C for 48 h.
The supernatant was decanted and the starch layer was re-slurried with water.