This review focuses on the current state of HPLC methods for the determination of vitamin C in food. The data for this review were collected from the Scopus database, in the period of time 2000–2014. After a brief summary of vitamin C characteristics, a critical discussion is presented regarding method development and optimization and, especially, method validation. The approaches used by different authors are discussed, mentioning the most important aspects to consider, the advantages and disadvantages of the analytical methods currently available, and future trends in vitamin C quantification and validation.