%Inhibition biofilm : Form the graph of %inhibition bioflim we can conclude that crude non-sonicate. crude sonicate and F2 non sonicate can inhibit biofilm formation of Escherichia coil and Staphylococcus aureus
%Inhibition growth : Crude non sonicate and crude sonicate can inhibit biofilm formation and cell growth. So they inhibit biofilm in growth pathway
F2 non sonicate can inhibit biofilm formation. but can inhibit cell growth . so it inhibit biofilm in surface attachment pathway.
Conclusion
Different collecting method contain higher bioactivity inhibiting the bacterial biofilm in different gram
The best fraction for S.aureus is Crude non-ultrasonicating sample
(IC50 – 203.78µg/ml)
The best fraction for E.coli is Crude of ultrasonicating sample
(IC50 = 333.36µg/ml)