Epithelioma papulosum cyprini cells (EPCs) (ATCC: CRL-2872)
which have a broad sensitivity for fish viruses such as Spring
viremia of carp virus were cultured in Eagle’s minimum essential
medium (MEM, Sigma) supplemented with penicillin (100 mg/ml),
streptomycin (100 mg/ml) and 10% calf bovine serum (FBS, Gibco) at
25 C. SVCV (ATCC: VR-1390) used for infection,