An aliquot of 50 μL of coffee sample diluted with demineralized water (5:1000 to 15:1000) was added to 2 mL of ABTS + reagent and the absorbance was monitored for 18 min at 25 °C.
An aliquot of 50 μL of coffee sample diluted with demineralized water (5:1000 to 15:1000) was added to 2 mL of ABTS reagent and the absorbance was monitored for 18 min at 25 °C.