Host range determination and efficiency of phage infection
The spot test was performed with the twelve bacteria to assess the
bacterial susceptibility to the bacteriophage (Vieira et al., 2012). The
plates were incubated at 37 °C for 4–12 h. The efficiency of plating
was determined for the bacteria with positive spot tests (occurrence
of a lysis zone) by the double-layer agar method using TSA as culture
medium. Efficacy of plating for each host was calculated by comparison
with an efficacy of 100% for the V. parahaemolyticus bacterium. For each
phage, three independent experiments were done and the results were
presented as the average of the three assays.