Influence of light on immature stages
To compare immature life-history traits
between the sunlight and artificial light
treatments, the cardboard rolls containing
eggs were placed in different 0.5-L glass
containers. Each container was covered with a
paper towel that was held in place with a
rubber band and stored in a greenhouse at 28°
C and 60% RH until larvae emerged. The
larvae were reared using a saturated grain diet
(Hogsette 1992). To differentiate life stages,
prepupae had a characteristic black cuticle
whereas larvae were white, and larval
development time was the time from the
appearance of the first larvae to the
appearance of the first prepupae. Pupal
development included the time from the
appearance of prepupae to the first adult
emergence. The sample sizes were 100 for
each cohort.