2.4. Cotton transformation
The hypocotyl explants were co-cultivated with bacterial culture
prepared in Agrobacterium minimal medium supplemented
with 100 lM Acetosyringone. Half-MS medium and
Cefotaxime (300 mg/ml) was used for washing of co-cultivated
explants and further cultured on MS medium containing
growth hormone 2,4-D (0.5 mg/l) and BAP (0.1 mg/l). After
4 weeks, the explants were further sub-cultured on MS medium
for somatic embryo induction till 6 weeks. The resistant
somatic embryos were sub-cultured in a MS medium for elongation.
The elongated somatic embryos were cultured on MS
with BAP and GA3 medium for germination. The germinated
seedling were hardened well and transferred to bigger pots and
finally shifted to greenhouse under natural condition.