In this study we employed our previously developed method for PCR
amplification and sequencing of the entire cps gene clusters of Campylobacter
(Karlyshev et al., 2005). The procedure is based on a combination
of primers corresponding to the flanking (highly conserved) regions with
those derived from the internal biosynthetic region. Previous studies
demonstrated that, despite overall high variability of the latter, some biosynthetic
genes may be present in different cps loci (Karlyshev et al.,
2005). The technique has led to the identification and sequencing of cps
regions of several other Campylobacter strains (Karlyshev et al., 2005;
Poly et al., 2011).
The conserved cps-related genes of strain X were detected using 30