HepG2 cells were cultured in a 6-well plate and treated with various concentrations of MLE. After treatments, cells were
washed three times with iced PBS and fixed with 4% paraformalin for 60 min. The cells were stained with 1 lg/ml Nile red
for 5 min in phosphate-buffer saline. To quantify Nile red content levels, flow cytometry was performed at an excitation
wavelength of 488 nm