In this study, we established a high efficiency micropropagation
protocol for Aloe vera and improved it even further by
adding spermidine. This optimized protocol led to the production
of more than 40 plants from one meristem of Aloe
vera, demonstrating much higher efficiency than previous
reported protocols (Abadi and Kaviani 2010; Hashemabadi
and Kaviani 2008; Liao et al. 2004; Meyer and Staden 1991;
Singh and Sood 2009; Du et al. 2004). In addition, regenerated
plants were easily transferred to soil without any loss and
grew normally without chromosome-level somaclonal variation.
This optimized protocol provides a valuable alternative
for mass propagation of elite Aloe vera.