3. Results and discussion
The concentration of four monosaccharides and five inhibitors were measured before and after cultivation to evaluate the consumption of sugars and changes in the concentrations of fermentation inhibitors during cultivation. The concentration of four monosaccharides and five inhibitors were also measured for hot water extraction before and after autoclaving. The analysis of monosaccharides is summarized in Table 2. The cultivations in wood hot water extract (HWE) without autoclaving did not resultin any substantial cellulose activity and this result was some what surprising, since it is not clear that the media contained compounds that inhibit Acetobacter xylinus 23769. This could have occurred due to contamination of the Hot water extract, which was not sterilized. However,in the Uraki, Morito, Kishimoto, and Sano (2002) study, bacterial cellulose production was not observed in the unpurified water soluble fraction (WS) of waste liquor from atmospheric acetic acidpulping due to presence of antibacterial activity in the unpurified water soluble . Bacterial cellulose production was only observed after purifying the medium with activated carbon. There fore, wood hot water extract should also be studied in terms of antibacterial activity. Monosaccharide concentration also did not change significantly before and after autoclaving. Cultures at pH 5 and 26 and 30◦C and at pH 6 and 26◦C did not produce bacterial cellulose and the reason why bacterial cellulose is not produced at these conditions requires further investigation. The results in Table 2 indicate that glucose and xylose were the main nutrient sources in all cultivations. The consumption of arabinose and fucose was negligible and it was also reported that Acetobacter xylinum strains have poor capability to utilize arabinose (Guo et al., 2013).
3. Results and discussionThe concentration of four monosaccharides and five inhibitors were measured before and after cultivation to evaluate the consumption of sugars and changes in the concentrations of fermentation inhibitors during cultivation. The concentration of four monosaccharides and five inhibitors were also measured for hot water extraction before and after autoclaving. The analysis of monosaccharides is summarized in Table 2. The cultivations in wood hot water extract (HWE) without autoclaving did not resultin any substantial cellulose activity and this result was some what surprising, since it is not clear that the media contained compounds that inhibit Acetobacter xylinus 23769. This could have occurred due to contamination of the Hot water extract, which was not sterilized. However,in the Uraki, Morito, Kishimoto, and Sano (2002) study, bacterial cellulose production was not observed in the unpurified water soluble fraction (WS) of waste liquor from atmospheric acetic acidpulping due to presence of antibacterial activity in the unpurified water soluble . Bacterial cellulose production was only observed after purifying the medium with activated carbon. There fore, wood hot water extract should also be studied in terms of antibacterial activity. Monosaccharide concentration also did not change significantly before and after autoclaving. Cultures at pH 5 and 26 and 30◦C and at pH 6 and 26◦C did not produce bacterial cellulose and the reason why bacterial cellulose is not produced at these conditions requires further investigation. The results in Table 2 indicate that glucose and xylose were the main nutrient sources in all cultivations. The consumption of arabinose and fucose was negligible and it was also reported that Acetobacter xylinum strains have poor capability to utilize arabinose (Guo et al., 2013).
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