Cultivation of C. militaris was conducted according to our
published method (Dong et al. 2012a), with some modifications.
Namely, after the media were covered by white
mycelia, they were transferred to lighting culture with the
temperature of 20C, and lightening of 400 lux for 12 h each
day. Two types of light-emitting diode (LED) lamps were
employed for lightening treatment: red light LED (emits
light of wavelength 620–630 nm) and blue light LED (emits
light of wavelength 440–450 nm). The red light lamp was
composed of 60 red light LEDs, with a power of 1.5 W. The
blue light lamp was composed of 60 blue light LEDs, with a
power of 1.5 W. Pink light lamp was composed of 40 red
light LEDs and 20 blue light LEDs, with a power of 1.5 W.
After 10 days lighting culture, all bottles were unsealed, with
the temperature of 25C in the day and 20C at night, humidity
of 85%, lightening of 400 lux for 12 h each day.
After lighting culture for 60 days, stick-shaped fruit body
grew up to 3 ~ 8 cm long (Fig 1). After the club-shaped fruit
bodies were covered with golden-yellow spores, the fruit
bodies were harvested. The harvested fruit bodies were
vacuum dried at 60C to constant weight for further analysis.