with some modifications. Filtrate was centrifuged at 8800 g for 15 min,the super-natant was discarded and the cell pellet
was recovered.Dimethyl sulfoxide (DMSO) was subsequently added for cell wall disruption,mixture was heatedat 60 C for 30 min with periodic vortex homogenization.Mixture was then treated with acetone ,vortexed and centrifuged.Total carotenoid concentrations were quantified spectrophotometrically at an