• Method for characterizing and fingerprinting
foodborne organisms
-DNA Amplification (polymerase chain reaction;
PCR)
*employ thermostable DNA polymerase,
specific nucleotides primer
*single molecule of DNA can be amplified to
10 107 within 20 20-50 cycles
*amplified DNA is detected by agarose gel or
southern blotting either radiolabeled or non non-
labeled probe