2.4. Analysis of crude fat, crude protein and water-soluble protein
The crude fat and nitrogen content was determined
according to AOAC official methods 920.39 and 954.01 (AOAC,
1997). Crude protein content was calculated by multiplying the
nitrogen content by 5.30 (FAO, 1986). Chestnut powder was
extracted by using distilled water three times, and the watersoluble
protein content was measured by using Coomassie Brilliant
Blue (Bradford, 1976). Bovine serum albumin (BSA) was used as the
standard.