ll microorganisms used for bioassay
were purchased from American Type Culture
Collection (Rockville, Maryland). The following
bacteria were used; S. aweus ATCC 6538,
P. aeruginosa ATCC 25619, and C. ahcans ATCC
18804. The activities were determined using the
macro dilution broth culture method. The
samples were dissolved in DMF. HeLa, ATCC
CCL2, cells were also purchased from ATCC. The
procedure for the cytotoxicity assay followed the
literature (6)